Transient histone deacetylase inhibition induces cellular memory of gene expression and 3D genome folding
ESC culture E14GT2a p14 cells were purchased from MMRRC, UC Davis. Pcgf4−/−; Pcgf2fl/fl cells were gift from R. Klose (University of Oxford), Pcgf4 deletion and Pcgf2 excision in response to tamoxifen (OHT) were verified by genotyping PCR. CTCF–AID–eGFP cells were a gift from R. Saldana-Meyer (Howard Hughes Medical Institute); CTCF–AID–eGFP expression was confirmed by anti-GFP